Screening Libraries

shRNA screening

The HTS Facility maintains and distributes clones and pools of plasmid or replication-incompetent lentivirus particles from the human and mouse pGIPZ library. For more information about the libraries refer to the GE Healthcare website

An online searchable database and ordering system called the RNAi Storefront at http://monod.fhcrc.org/rnai/ has been established to facilitate purchase of individual clones, pooled plasmid library sets, gene family sub-library pools, pre-packaged lentivirus pools, and various controls. Browsing is unrestricted, however, you will be prompted for your HutchNet ID and password to submit an order. Please contact hutchbase-support@fredhutch.org with access or content issues. Please note that access to the shRNA collections is limited to Fred Hutch labs only.

siRNA screening

The HTS facility maintains and manages the following human and mouse siRNA libraries for well-by-well RNAi screening experiments:

  • Qiagen Human Druggable Genome
    6961 genes targeted by 27844 unique triggers on 89 96-well plates or 23 384-well plates
  • Qiagen Mouse Druggable Genome
    8353 genes targeted by 33412 unique triggers on 102 96-well plates or 26 384-well plates
  • Qiagen Human Transcription Factor
    2254 genes targeted by 9016 unique triggers on 29 96-well plates or 8 384-well plates
  • Qiagen Mouse Transcription Factor
    1675 genes targeted by 6700 unique triggers on 18 96-well plates or 5 384-well plates
  • Ambion Human Kinase
    2130 genes targeted by 710 unique triggers on 27 96-well plates or 7 384-well plates

General information on the Qiagen siRNA libraries is available from the Qiagen web site or the Ambion web site

Small molecule libraries

The HTS facility maintains and manages the following small molecule libraries for well-by-well drug screening experiments:

ChemDiv website: https://www.chemdiv.com/ 

MicroSource website: http://www.msdiscovery.com/