DNA/RNA QC TapeStation

Contact: Elizabeth Jensen
Location: Thomas Bldg DE-740
Contact phone: (206) 667-4470
Contact fax: (206) 667-2825
Contact e-mail: ejensen@fredhutch.org

Lab Introduction and Consultation

The current platform used in the resource for qualitative and semi-quantitative analysis of DNA and RNA is the Agilent 4200 TapeStation. For an overview of the Agilent 4200 TapeStation, please visit the Agilent website.

The Genomics Shared Resource currently provides TapeStation services for nucleic acid applications. Those new to this technology or new to using the Resource are strongly encouraged to meet with lab personnel prior to planning a project. For non-consortium members interested in services please contact the Genomics Shared Resource at (206)-667-2714.

Service Request and Sample Submission (Fred Hutch)

For Fred Hutch-based labs, services are requested through HutchBASE, where the requester is asked to input sample names, concentrations, project ID number, and assay specific information. Please use strip tubes or 96-well plates loaded vertically A-H. Please refer to this document (link to PDF) for detailed instructions on sample submission and data retrieval.

Samples must be submitted to the DNA Array Lab (DE-740) in volumes ≥ 3ul and at a concentration appropriate for the desired assay (please refer to the guidelines in HutchBASE). You will receive an automated email once your data is available that will direct you to your results in HutchBASE. Please inquire with resource personnel for more details.

Service Request and Sample Submission (External)

If billing is to be submitted to the University of Washington or other external institution, please fill out the External Submission Form.

Samples and a fully completed sample submission form should be delivered to the DNA Array Lab, located in the Thomas Building, DE-740.  Please call the resource in advance to schedule a delivery (206.667.2714).

Image and Data from Agilent 2200 TapeStation

Results are generated with the Agilent 4200 TapeStation Software in the form of a pdf document. These documents present data in peak tables, pseudo-gel images, electropherograms, and an assay-specific results table. Raw data files will be supplied upon request. In this case the researcher will need to download a trial version of the 4200 TapeStation Software from the Agilent website.