10x Genomics Single Cell

Contact: Jeff Delrow
Location: Thomas Bldg DE-740
Contact phone: (206) 667-2714
Contact fax: (206) 667-2825
Contact e-mail: jdelrow@fredhutch.org

The Genomics & Bioinformatics Shared Resource (G&BSR) is supporting single cell library construction using 10x Genomics Chromium technology.  For an overview of the technology, we invite you to visit the 10x Genomics website.

The list of current assays available using the 10x Genomics Chromium platform include:

The Chromium Single Cell Gene Expression solution allows for high-throughput single cell expression profiling of 3’ transcripts covering 500-10,000 individual cells per sample.

The Chromium Single Cell Immune Profiling solution simultaneously allows the study of cellular context of the adaptive immune response and the immune repertoires of hundreds to millions of individual T and B cells.

The Chromium Exome Sequencing solution provides long-range information, thereby enabling phasing, structural variant detection, and copy number determination.

Additional and future single-cell assays can be found on the 10X Genomics website.

There are two options for accessing 10x Genomics technology through our facility.

  1. Self-Use Option. For internal users trained by a certified 10x Genomics field application specialist, you have the option of using the system independently. Please note that proof of training will be required. Noncertified users run the risk of voiding any warantee coverage should an issue arise while operating the controller.
  2. Full-Service Option. Arrangements can be made with Genomics staff to perform single cell isolation and library preparation using the 10x Genomics system.