The yeast alpha-factor receptor: structural properties deduced from the sequence of the STE2 gene.

Publication Type:

Journal Article


Nucleic acids research, Volume 13, Issue 23, p.8463-75 (1985)


Amino Acid Sequence, Base Sequence, DNA, Fungal, Fungal Proteins, Genes, Fungal, Genetic Complementation Test, Membrane Proteins, Peptides, Pheromones, Protein Conformation, Receptors, Cell Surface, Saccharomyces cerevisiae, Solubility


The STE2 gene of the yeast Saccharomyces cerevisiae encodes a component of the receptor for the oligopeptide pheromone alpha-factor. We have cloned and determined the nucleotide sequence of the STE2 gene. A sequence involved in the control of cell-type expression of the STE2 gene was found 5' of an open reading frame that could encode a protein of 431 amino acids. The predicted STE2 protein contains seven hydrophobic segments, suggesting that the alpha-factor receptor is an integral membrane protein. No extensive homology at the primary sequence level was detected between the predicted STE2 protein and other available protein sequences.