The yeast alpha-factor receptor: structural properties deduced from the sequence of the STE2 gene.

Publication Type:

Journal Article

Source:

Nucleic acids research, Volume 13, Issue 23, p.8463-75 (1985)

Keywords:

Amino Acid Sequence, Base Sequence, DNA, Fungal, Fungal Proteins, Genes, Fungal, Genetic Complementation Test, Membrane Proteins, Peptides, Pheromones, Protein Conformation, Receptors, Cell Surface, Saccharomyces cerevisiae, Solubility

Abstract:

The STE2 gene of the yeast Saccharomyces cerevisiae encodes a component of the receptor for the oligopeptide pheromone alpha-factor. We have cloned and determined the nucleotide sequence of the STE2 gene. A sequence involved in the control of cell-type expression of the STE2 gene was found 5' of an open reading frame that could encode a protein of 431 amino acids. The predicted STE2 protein contains seven hydrophobic segments, suggesting that the alpha-factor receptor is an integral membrane protein. No extensive homology at the primary sequence level was detected between the predicted STE2 protein and other available protein sequences.