Using Schizosaccharomyces pombe meiosis to analyze DNA recombination intermediates.

Publication Type:

Journal Article

Source:

Methods in molecular biology (Clifton, N.J.), Volume 557, p.235-52 (2009)

Keywords:

2009, Basic Sciences Division, Cell Culture Techniques, DNA, Fungal, Electrophoresis, Agar Gel, MEIOSIS, Models, Biological, Recombination, Genetic, Schizosaccharomyces

Abstract:

The fission yeast Schizosaccharomyces pombe has many biological characteristics that make it an ideal model organism for the study of meiosis. A nearly synchronous meiosis is one of the most important. Under certain environmental and genetic conditions, large cultures of S. pombe can be induced to undergo meiosis in a timely and predictable manner that allows for changes in the DNA to be observed and analyzed by gel electrophoresis. Initiation of meiotic recombination via programmed DNA double-strand breaks, the formation of joint molecule recombination intermediates, and the resolution of these intermediates into crossover DNA products can all be seen with consistent timing during the progression of a synchronous meiotic induction. The timing of recombination events, the genetic requirements for the formation and disappearance of recombination intermediates, and the analysis of the DNA structures of those intermediates allow a comparison of meiotic recombination in fission yeast with that in the only other species similarly studied, the budding yeast Saccharomyces cerevisiae.