Use of yeast-secreted in vivo biotinylated recombinant antibodies (Biobodies) in bead-based ELISA.

Publication Type:

Journal Article


Clinical cancer research : an official journal of the American Association for Cancer Research, Volume 14, Issue 9, p.2647-55 (2008)


2008, Adult, Aged, Aged, 80 and over, Antibodies, Antibodies, Monoclonal, BIOTINYLATION, Center-Authored Paper, Enzyme-Linked Immunosorbent Assay, Epididymal Secretory Proteins, Female, GPI-Linked Proteins, Humans, Membrane Glycoproteins, Middle Aged, Ovarian Neoplasms, Protein Engineering, Public Health Sciences Division, Recombinant Fusion Proteins, Reproducibility of Results, Sensitivity and Specificity, Shared Resources, Specimen Processing Core Facility, Yeasts


To measure circulating antigens, sandwich ELISA assays require two complementary affinity reagents. Mouse monoclonal antibodies (mAb) and polyclonal antibodies (pAb) are commonly used, but because their production is lengthy and costly, recombinant antibodies are emerging as an attractive alternative.