Transmission and expansion of HOXB4-induced leukemia in two immunosuppressed dogs: implications for a new canine leukemia model.

Publication Type:

Journal Article

Source:

Experimental hematology, Volume 37, Issue 10, p.1157-66 (2009)

Keywords:

2009, Accidents, Animals, Biologics Production Core Facility, Catheterization, Cell Line, Tumor, Center-Authored Paper, Clinical Research Division, Comparative Medicine Core Facility, Disease Models, Animal, Dogs, Equipment Contamination, Experimental Histopathology Core Facility, Flow Cytometry Core Facility, Fluid Therapy, Genes, Reporter, Genetic Vectors, Green Fluorescent Proteins, hematopoietic stem cell transplantation, Histocompatibility Antigens Class I, Homeodomain Proteins, Immunocompromised Host, Immunosuppressive Agents, Leukemia, Myeloid, Minisatellite Repeats, Neoplasm Transplantation, Neoplastic Stem Cells, Radiation Chimera, Recombinant Fusion Proteins, Research Trials Office Core Facility - Biostatistics Service, Shared Resources, TRANSCRIPTION FACTORS

Abstract:

There are currently no large animal models to study the biology of leukemia and development of novel antileukemia therapies. We have previously shown that dogs transplanted with homeobox B4 (HOXB4)-transduced autologous CD34(+) cells developed myeloid leukemia associated with HOXB4 overexpression. Here we describe the transmission, engraftment, and expansion of these canine leukemia cells into two genetically unrelated, immunosuppressed dogs.