A transmissible retrovirus expressing human hypoxanthine phosphoribosyltransferase (HPRT): gene transfer into cells obtained from humans deficient in HPRT.

Publication Type:

Journal Article

Source:

Proceedings of the National Academy of Sciences of the United States of America, Volume 80, Issue 15, p.4709-13 (1983)

Keywords:

363, Animals, Cell Line, Cloning, Molecular, DNA, GENES, Genes, Viral, Helper Viruses, Humans, Hypoxanthine Phosphoribosyltransferase, MICE, Mice, Inbred BALB C, Rats, Rats, Inbred F344, Retroviridae, Simian virus 40, Transfection

Abstract:

A cDNA corresponding to the human gene for hypoxanthine phosphoribosyltransferase (HPRT; IMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.8) has been ligated into murine retroviral vectors such that it is under the transcriptional control of viral long terminal repeats. Transfection of HPRT- cells followed by superinfection with various helper viruses has led to the rescue of chimeric virus capable of transmitting the HPRT+ phenotype to HPRT- rodent or human cells. These genetically transformed cells contain authentic human HPRT at levels similar to normal HPRT+ cells.