Transformation and scattering activities of the receptor tyrosine kinase RON/Stk in rodent fibroblasts and lack of regulation by the jaagsiekte sheep retrovirus receptor, Hyal2.

Publication Type:

Journal Article

Source:

BMC cancer, Volume 4, p.64 (2004)

Keywords:

Animals, Cell Adhesion Molecules, Cell Transformation, Viral, Fibroblasts, GPI-Linked Proteins, Humans, Hyaluronoglucosaminidase, Membrane Proteins, MICE, NIH 3T3 Cells, PHENOTYPE, Rats, Receptor Protein-Tyrosine Kinases, Receptors, Virus, Recombinant Proteins, Serine Endopeptidases, Transfection

Abstract:

The envelope (Env) protein of jaagsiekte sheep retrovirus (JSRV) can transform cells in culture and is likely to be the main factor responsible for lung cancer induction by JSRV in animals. A recent report indicates that the epithelial-cell transforming activity of JSRV Env depends on activation of the cell-surface receptor tyrosine kinase Mst1r (called RON for the human and Stk for the rodent orthologs). In the immortalized line of human epithelial cells used (BEAS-2B cells), the virus receptor Hyal2 was found to bind to and suppress the activity of RON. When Env was expressed it bound to Hyal2 causing its degradation, release of RON activity from Hyal2 suppression, and activation of pathways resulting in cell transformation.