TILLING to detect induced mutations in soybean.

Publication Type:

Journal Article

Source:

BMC plant biology, Volume 8, p.9 (2008)

Keywords:

2008, Base Sequence, Basic Sciences Division, Center-Authored Paper, DNA Primers, DNA, Plant, Ethyl Methanesulfonate, Genomics Core Facility, Methylnitrosourea, mutagenesis, Shared Resources, Soybeans

Abstract:

Soybean (Glycine max L. Merr.) is an important nitrogen-fixing crop that provides much of the world's protein and oil. However, the available tools for investigation of soybean gene function are limited. Nevertheless, chemical mutagenesis can be applied to soybean followed by screening for mutations in a target of interest using a strategy known as Targeting Induced Local Lesions IN Genomes (TILLING). We have applied TILLING to four mutagenized soybean populations, three of which were treated with ethyl methanesulfonate (EMS) and one with N-nitroso-N-methylurea (NMU).