RNA interference in mammalian cell systems.

Publication Type:

Journal Article


Current topics in microbiology and immunology, Volume 320, p.1-19 (2008)


Animals, Eukaryotic Initiation Factors, Gene Library, Genetic Techniques, GENOME, Humans, MAMMALS, Ribonuclease III, RNA Interference


The use of RNA interference (RNAi) to evoke gene silencing in mammalian cells has almost become routine laboratory practice. Through refinement of double-stranded RNA (dsRNA) triggers of RNAi and creation of genome-scale libraries, the first genome-wide loss of function screens have been carried out in mammals. This review discusses some of the key features of RNAi in mammalian systems.