RNA interference in mammalian cell systems.

Publication Type:

Journal Article

Source:

Current topics in microbiology and immunology, Volume 320, p.1-19 (2008)

Keywords:

Animals, Eukaryotic Initiation Factors, Gene Library, Genetic Techniques, GENOME, Humans, MAMMALS, Ribonuclease III, RNA Interference

Abstract:

The use of RNA interference (RNAi) to evoke gene silencing in mammalian cells has almost become routine laboratory practice. Through refinement of double-stranded RNA (dsRNA) triggers of RNAi and creation of genome-scale libraries, the first genome-wide loss of function screens have been carried out in mammals. This review discusses some of the key features of RNAi in mammalian systems.