Reverse genetics in zebrafish by TILLING.

Publication Type:

Journal Article


Briefings in functional genomics & proteomics, Volume 7, Issue 6, p.454-9 (2008)


2008, Animals, Base Pair Mismatch, Basic Sciences Division, Center-Authored Paper, Comparative Medicine Core Facility, Ethylnitrosourea, Models, Biological, mutagenesis, Mutagens, Shared Resources, zebrafish


TILLING, for Targeting Induced Local Lesions in Genomes, is a reverse genetics strategy that identifies mutations in specific genes of interest in chemically mutagenized populations. First described in 2000 for mutation detection in Arabidopsis, TILLING is now used in a wide range of plants including soybean, rice, barley and maize as well as for animal model systems, including Arabidopsis, Drosophila, Caenorhabditis elegans, rat, medaka and zebrafish and for the discovery of naturally occurring polymorphisms in humans. This review summarizes current TILLING methodologies as they have been applied to the zebrafish, ongoing TILLING projects and resources in the zebrafish community, and the future of zebrafish TILLING.