Retroviral transfer of genes into canine hemopoietic progenitor cells in culture: a model for human gene therapy.

Publication Type:

Journal Article

Source:

Proceedings of the National Academy of Sciences of the United States of America, Volume 83, Issue 12, p.4552-5 (1986)

Keywords:

1986, Animals, Bone Marrow Cells, Cells, Cultured, Dogs, Genetic Engineering, Genetic Vectors, Granulocytes, Hematopoietic Stem Cells, Macrophages, Retroviridae, Tetrahydrofolate Dehydrogenase, Time Factors

Abstract:

Amphotropic retroviral vectors containing either a mutant dihydrofolate reductase gene (DHFR) or the bacterial neomycin phosphotransferase gene (neo) were used to infect canine hemopoietic cells. We report successful transfer and expression of the DHFR and neo genes in canine hemopoietic progenitor cells (colony-forming units, granulocyte/macrophage) as measured by the ability of the viruses to confer resistance to either methotrexate or the aminoglycoside G418, respectively. Transfer was achieved in the absence of helper virus by using retrovirus packaging cell lines. Successful transfer of these genes into canine hemopoietic progenitor cells in vitro indicates the feasibility of gene transfer into canine marrow for autologous reconstitution. Studies of transfer of new genetic information into a large, outbred animal such as the dog will provide a preclinical model for future gene therapy in humans.