Replication in a superficial epithelial cell niche explains the lack of pathogenicity of primate foamy virus infections.

Publication Type:

Journal Article

Source:

Journal of virology, Volume 82, Issue 12, p.5981-5 (2008)

Keywords:

2008, Animals, Basic Sciences Division, Center-Authored Paper, DNA, Viral, Epithelial Cells, Experimental Histopathology Core Facility, In Situ Hybridization, Macaca mulatta, Monkey Diseases, Retroviridae Infections, RNA, Viral, Shared Resources, Spumavirus, Virus Replication

Abstract:

Foamy viruses (FVs) are ancient retroviruses that are ubiquitous in nonhuman primates (NHPs). While FVs share many features with pathogenic retroviruses, such as human immunodeficiency virus, FV infections of their primate hosts have no apparent pathological consequences. Paradoxically, FV infections of many cell types in vitro are rapidly cytopathic. Previous work has shown that low levels of proviral DNA are found in most tissues of naturally infected rhesus macaques, but these proviruses are primarily latent. In contrast, viral RNA, indicative of viral replication, is restricted to tissues of the oral mucosa, where it is abundant. Here, we perform in situ hybridization on tissues from rhesus macaques naturally infected with simian FV (SFV). We show that superficial differentiated epithelial cells of the oral mucosa, many of which appear to be shedding from the tissue, are the major cell type in which SFV replicates. Thus, the innocuous nature of SFV infection can be explained by replication that is limited to differentiated superficial cells that are short-lived and shed into saliva. This finding can also explain the highly efficient transmission of FVs among NHPs.