Removal of a 67-base-pair sequence in the noncoding region of protooncogene fos converts it to a transforming gene.

Publication Type:

Journal Article


Proceedings of the National Academy of Sciences of the United States of America, Volume 82, Issue 15, p.4987-91 (1985)


361, Animals, Base Sequence, Cell Transformation, Viral, Chromosome Deletion, Gene Expression Regulation, MICE, Mutation, Nucleic Acid Conformation, Oncogenes, Poly A, Repetitive Sequences, Nucleic Acid, Sarcoma Viruses, Murine


Transformation of fibroblasts by protooncogene fos (c-fos) requires the linkage of viral long terminal repeat (LTR) sequences and interruption of 3'-noncoding sequences. We have identified an A + T-rich stretch of 67 nucleotides, located 627-693 base pairs downstream from the coding domain and 123-189 base pairs upstream from the putative poly(A) addition site, removal of which confers transforming activity to the c-fos gene. A novel regulation of the expression of the c-fos gene is proposed, which may be functional in vivo to prevent the gene from becoming an oncogene.