Removal of a 67-base-pair sequence in the noncoding region of protooncogene fos converts it to a transforming gene.

Publication Type:

Journal Article

Source:

Proceedings of the National Academy of Sciences of the United States of America, Volume 82, Issue 15, p.4987-91 (1985)

Keywords:

361, Animals, Base Sequence, Cell Transformation, Viral, Chromosome Deletion, Gene Expression Regulation, MICE, Mutation, Nucleic Acid Conformation, Oncogenes, Poly A, Repetitive Sequences, Nucleic Acid, Sarcoma Viruses, Murine

Abstract:

Transformation of fibroblasts by protooncogene fos (c-fos) requires the linkage of viral long terminal repeat (LTR) sequences and interruption of 3'-noncoding sequences. We have identified an A + T-rich stretch of 67 nucleotides, located 627-693 base pairs downstream from the coding domain and 123-189 base pairs upstream from the putative poly(A) addition site, removal of which confers transforming activity to the c-fos gene. A novel regulation of the expression of the c-fos gene is proposed, which may be functional in vivo to prevent the gene from becoming an oncogene.