Proteomic global profiling for cancer biomarker discovery.

Publication Type:

Journal Article

Source:

Methods in molecular biology (Clifton, N.J.), Volume 492, p.309-20 (2009)

Keywords:

2009, Acrylamide, Blood Chemical Analysis, Blood Proteins, Center-Authored Paper, Chemical Fractionation, Chromatography, Liquid, Humans, Isotope Labeling, mass spectrometry, Protein Isoforms, PROTEOMICS, Public Health Sciences Division, Tumor Markers, Biological

Abstract:

The ultimate goal of cancer molecular diagnostics is the development of simple tests to predict cancer risk, detect cancer early, classify tumors, and monitor response to therapy. Proteomics is well suited for these tasks. However, there are substantial challenges that need to be met to identify the most informative markers using proteomics. Approaches for in-depth quantitative proteomic analysis based on isotopic labeling and protein fractionation are presented in this chapter.