Proteomic analysis of ovarian cancer cells reveals dynamic processes of protein secretion and shedding of extra-cellular domains.

Publication Type:

Journal Article

Authors:

Faça, Vitor M; Ventura, Aviva P; Fitzgibbon, Mathew P; Pereira-Faça, Sandra R; Pitteri, Sharon J; Green, Ann E; Ireton, Renee C; Zhang, Qing; Wang, Hong; O'Briant, Kathy C; Drescher, Charles W; Schummer, Michèl; McIntosh, Martin W; Knudsen, Beatrice S; Hanash, Samir M

Source:

PloS one, Volume 3, Issue 6, p.e2425 (2008)

Keywords:

2008, Cell Line, Tumor, Center-Authored Paper, Chromatography, Liquid, Extracellular Space, Female, Humans, Neoplasm Proteins, Ovarian Neoplasms, Proteomics Core Facility, Public Health Sciences Division, Shared Resources, Tandem Mass Spectrometry

Abstract:

Elucidation of the repertoire of secreted and cell surface proteins of tumor cells is relevant to molecular diagnostics, tumor imaging and targeted therapies. We have characterized the cell surface proteome and the proteins released into the extra-cellular milieu of three ovarian cancer cell lines, CaOV3, OVCAR3 and ES2 and of ovarian tumor cells enriched from ascites fluid.