A preclinical model of double- versus single-unit unrelated cord blood transplantation.

Publication Type:

Journal Article

Source:

Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation, Volume 16, Issue 8, p.1090-8 (2010)

Keywords:

2010, Animals, Biologics Production Core Facility, Cell Processing Core Facility, Center-Authored Paper, Clinical Research Division, Comparative Medicine Core Facility, Cord Blood Stem Cell Transplantation, Disease Models, Animal, Dogs, Experimental Histopathology Core Facility, Female, Flow Cytometry Core Facility, Graft Rejection, Male, Research Trials Office Core Facility - Biostatistics Service, Shared Resources, Survival Rate

Abstract:

Cord blood transplantation (CBT) with units containing total nucleated cell (TNC) dose >2.5 x 10(7)/kg is associated with improved engraftment and decreased transplant-related mortality. For many adults no single cord blood units are available that meet the cell dose requirements. We developed a dog model of CBT to evaluate approaches to overcome the problem of low cell dose cord blood units. This study primarily compared double- versus single-unit CBT. Unrelated dogs were bred and cord blood units were harvested. We identified unrelated recipients that were dog leukocyte antigen (DLA)-88 (class I) and DLA-DRB1 (class II) allele-matched with cryopreserved units. Each unit contained