No telomere shortening in marrow stroma from patients with MDS.

Publication Type:

Journal Article

Source:

Annals of hematology, Volume 88, Issue 7, p.623-8 (2009)

Keywords:

2009, Adult, Age Factors, Aged, Aged, 80 and over, Bone Marrow, Case-Control Studies, Cells, Cultured, Center-Authored Paper, Clinical Research Division, Female, Flow Cytometry Core Facility, Hematopoietic Stem Cells, Humans, In Situ Hybridization, Fluorescence, Male, Middle Aged, Myelodysplastic Syndromes, Polymerase Chain Reaction, Shared Resources, Stromal Cells, Telomere

Abstract:

Telomere shortening with age may lead to genomic instability and an increased risk of cancer. Given the role of the microenvironment in the pathophysiology of the myelodysplastic syndrome (MDS), primarily a disease of older age, we determined telomere length in primary cultured marrow stroma cells using quantitative fluorescent in situ hybridization (qFISH) and quantitative polymerase chain reaction (qPCR). qFISH showed comparable rates of decrease in telomere length with age in MDS patients and age-matched healthy controls. Telomere length assessment by qPCR showed similar results. These findings suggest a lack of significant differences between MDS patients and healthy controls in terms of telomere stability in marrow stroma in contrast to that observed in hematopoietic cells. In conclusion, this demonstrates that, although MDS stroma cells and hematopoietic cells share the same microenvironment, the stromal cells do not share the processes that contribute to accelerated telomere attrition, suggesting that stromal cell proliferative potential is not limiting in MDS.