Multiple integrated copies and high-level production of the human retrovirus XMRV (xenotropic murine leukemia virus-related virus) from 22Rv1 prostate carcinoma cells.

Publication Type:

Journal Article

Source:

Journal of virology, Volume 83, Issue 14, p.7353-6 (2009)

Keywords:

2009, Animals, CARCINOMA, Cell Line, Tumor, Center-Authored Paper, Electron Microscopy Core Facility, Gene Dosage, Genomics Core Facility, Human Biology Division, Humans, Male, MICE, Phylogeny, Prostatic Neoplasms, Retroviridae, Shared Resources, Virus Integration

Abstract:

The human retrovirus XMRV (xenotropic murine leukemia virus-related virus) is associated with prostate cancer, most frequently in humans with a defect in the antiviral defense protein RNase L, suggesting a role for XMRV in prostate carcinogenesis. However, XMRV has not been found in prostate carcinoma cells. Here we show that 22Rv1 prostate carcinoma cells produce high-titer virus that is nearly identical in properties and sequence to XMRV isolated by others and consist primarily of a single clone of cells with at least 10 integrated copies of XMRV, warranting further study of a possible role for XMRV integration in carcinogenesis.