Maternal/Child HLA Class II Compatibility in Pregnancies Affected by Preeclampsia

Publication Type:

Journal Article

Source:

Reproductive Sciences, Volume 21, Number 3, p.303a-303a (2014)

Keywords:

2014, Clinical Research Division, Clinical Research Division June 2014 2014Q2 CCSG, June 2014

Abstract:

INTRODUCTION: Lack of immunogenetic diversity appears to be a risk factor for adverse pregnancy outcomes, including preeclampsia (PE), and both epidemiologic and laboratory data suggest that immune dysfunction contributes to PE pathophysiology. We sought to examine maternal and child human leukocyte antigen (HLA) Class II relationships in PE compared with normal pregnancy, hypothesizing increased HLA compatibility in PE pregnancies. METHODS: DNA was isolated from blood or buccal swab samples from mother/child pairs. HLA typing for the Class II loci DRB1, DQA1, and DQB1 was performed using a polymerase chain reaction (pCR)-sequence specific oligonucleotide probe technique. Maternal and child HLA relationships were examined to determine low-resolution compatibility, considering both bidirectional compatibility and unidirectional compatibility which can occur in the setting of maternal or child homozygosity. Chi square was used for analysis. RESULTS: Results are summarized below. HLA Class II compatibility did not differ between mother/child pairs with (n=110) and without (n=108) PE. [results table] CONCLUSIONS: In our study, HLA Class II matemal/child compatibility did not differ in PE compared with normal pregnancy, however Class I was not explored and may be involved. Prior studies have suggested that paternal antigen exposure may alter which HLA relationships associate with PE, and it may be that other clinical factors also play a role. In addition, the classical definitions of HLA genotypes may be limited in their reflection of functionally important immunologic interactions, and alternative methods of evaluating HLA compatibility may provide useful information in future studies.

Notes:

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