Long-term expression of human adenosine deaminase in vascular smooth muscle cells of rats: a model for gene therapy.

Publication Type:

Journal Article

Source:

Proceedings of the National Academy of Sciences of the United States of America, Volume 89, Issue 3, p.1138-42 (1992)

Keywords:

1992, Adenosine Deaminase, Animals, gene expression, Gene Therapy, Genetic Vectors, Humans, Muscle, Smooth, Vascular, Rats, Retroviridae, RNA, Messenger, Transfection

Abstract:

Gene transfer into vascular smooth muscle cells in animals was examined by using recombinant retroviral vectors containing an Escherichia coli beta-galactosidase gene or a human adenosine deaminase (adenosine aminohydrolase, EC 3.5.4.4) gene. Direct gene transfer by infusion of virus into rat carotid arteries was not observed. However, gene transfer by infection of smooth muscle cells in culture and seeding of the transduced cells onto arteries that had been denuded of endothelial cells was successful. Potentially therapeutic levels of human adenosine deaminase activity were detected over 6 months of observation, indicating the utility of vascular smooth muscle cells for gene therapy in humans.