Jaagsiekte sheep retrovirus envelope efficiently pseudotypes human immunodeficiency virus type 1-based lentiviral vectors.

Publication Type:

Journal Article

Source:

Journal of virology, Volume 78, Issue 5, p.2642-7 (2004)

Keywords:

2004, Amino Acid Sequence, Animals, Cell Adhesion Molecules, Cell Division, Cells, Cultured, Epithelial Cells, Fibroblasts, Gene Products, env, Gene Therapy, Genetic Vectors, GPI-Linked Proteins, HIV-1, Human Biology Division, Humans, Hyaluronoglucosaminidase, Jaagsiekte sheep retrovirus, lung, Molecular Sequence Data, Moloney murine leukemia virus, Organ Specificity, Plasmids, Receptors, Virus, Recombinant Fusion Proteins, Rodentia, Transduction, Genetic

Abstract:

Jaagsiekte sheep retrovirus (JSRV) infects lung epithelial cells in sheep, and oncoretroviral vectors bearing JSRV Env can mediate transduction of human cells, suggesting that such vectors might be useful for lung-directed gene therapy. Here we show that JSRV Env can also efficiently pseudotype a human immunodeficiency virus type 1-based lentiviral vector, a more suitable vector for transduction of slowly dividing lung epithelial cells. We created several chimeric Env proteins that, unlike the parental Env, do not transform rodent fibroblasts but are still capable of pseudotyping lentiviral and oncoretroviral vectors.