Isolation of two genes that affect mitotic chromosome transmission in S. cerevisiae.

Publication Type:

Journal Article


Cell, Volume 44, Issue 1, p.53-63 (1986)


aneuploidy, Chromosome Mapping, Cloning, Molecular, DNA, Fungal, Gene Expression Regulation, Genes, Fungal, Mitosis, Recombination, Genetic, RNA, Fungal, Saccharomyces cerevisiae


Two DNA sequences that reduce mitotic fidelity of chromosome transmission have been identified: MIF1 and MIF2. MIF1 is a unique sequence located on the right arm of chromosome XII that stimulates loss and recombination for both chromosomes V and VII when present in a high copy number plasmid. MIF1 is not essential for cell division but is necessary for the normal fidelity of chromosome transmission. MIF2 is a unique sequence located 15 cM distal to HIS6 on chromosome IX that induces a high frequency of chromosome VII loss and a lower frequency of chromosome V loss when present in high copy number; it has no effect on mitotic recombination. Disruption of the genomic MIF2 locus was lethal and cells lacking this function arrested division with a terminal phenotype characteristic of a block in DNA replication or nuclear division.