Isolation of two genes that affect mitotic chromosome transmission in S. cerevisiae.

Publication Type:

Journal Article

Source:

Cell, Volume 44, Issue 1, p.53-63 (1986)

Keywords:

aneuploidy, Chromosome Mapping, Cloning, Molecular, DNA, Fungal, Gene Expression Regulation, Genes, Fungal, Mitosis, Recombination, Genetic, RNA, Fungal, Saccharomyces cerevisiae

Abstract:

Two DNA sequences that reduce mitotic fidelity of chromosome transmission have been identified: MIF1 and MIF2. MIF1 is a unique sequence located on the right arm of chromosome XII that stimulates loss and recombination for both chromosomes V and VII when present in a high copy number plasmid. MIF1 is not essential for cell division but is necessary for the normal fidelity of chromosome transmission. MIF2 is a unique sequence located 15 cM distal to HIS6 on chromosome IX that induces a high frequency of chromosome VII loss and a lower frequency of chromosome V loss when present in high copy number; it has no effect on mitotic recombination. Disruption of the genomic MIF2 locus was lethal and cells lacking this function arrested division with a terminal phenotype characteristic of a block in DNA replication or nuclear division.