Integrated proteomic analysis of human cancer cells and plasma from tumor bearing mice for ovarian cancer biomarker discovery.

Publication Type:

Journal Article

Source:

PloS one, Volume 4, Issue 11, p.e7916 (2009)

Keywords:

2009, Animals, Cell Line, Tumor, Center-Authored Paper, Enzyme-Linked Immunosorbent Assay, Female, Gene Expression Regulation, Neoplastic, Humans, Immunoblotting, mass spectrometry, MICE, Models, Statistical, Neoplasm Transplantation, Ovarian Neoplasms, Proteome, PROTEOMICS, Public Health Sciences Division, Tumor Markers, Biological

Abstract:

The complexity of the human plasma proteome represents a substantial challenge for biomarker discovery. Proteomic analysis of genetically engineered mouse models of cancer and isolated cancer cells and cell lines provide alternative methods for identification of potential cancer markers that would be detectable in human blood using sensitive assays. The goal of this work is to evaluate the utility of an integrative strategy using these two approaches for biomarker discovery.