Imputation of coding variants in African Americans: better performance using data from the exome sequencing project.

Publication Type:

Journal Article

Source:

Bioinformatics (Oxford, England), Volume 29, Issue 21, p.2744-9 (2013)

Keywords:

2013, Center-Authored Paper, Public Health Sciences Division, September 2013, Specimen Processing Core Facility

Abstract:

Although the 1000 Genomes haplotypes are the most commonly used reference panel for imputation, medical sequencing projects are generating large alternate sets of sequenced samples. Imputation in African Americans using 3384 haplotypes from the Exome Sequencing Project, compared with 2184 haplotypes from 1000 Genomes Project, increased effective sample size by 8.3-11.4% for coding variants with minor allele frequency <1%. No loss of imputation quality was observed using a panel built from phenotypic extremes. We recommend using haplotypes from Exome Sequencing Project alone or concatenation of the two panels over quality score-based post-imputation selection or IMPUTE2's two-panel combination.