Identification of recombinant human papillomavirus type 16 variants.

Publication Type:

Journal Article


Virology, Volume 394, Issue 1, p.8-11 (2009)


2009, Center-Authored Paper, DNA, Viral, Female, Human Biology Division, Human papillomavirus 16, Humans, Papillomavirus Infections, Polymerase Chain Reaction, Polymorphism, Genetic, Public Health Sciences Division, Recombination, Genetic, Sequence Analysis, DNA, Young Adult


Intratypic diversity of human papillomavirus (HPV) genome is generally characterized by point mutation, insertion, and/or deletion. Using PCR-based cloning and sequencing, we detected concurrent infection with 8 HPV16 variants in a woman enrolled in the ASCUS-LSIL Triage Study. The European variant was the major variant; each of the 7 minor variants had partial DNA sequences identical to the European variant and another part identical to the African 2 variant. At a follow-up visit, only an HPV16 African 2 variant was detected. Results from the present study suggest presence of intratypic recombination of HPV genome in natural infection.