Identification of the centromeric repeat in the threespine stickleback fish (Gasterosteus aculeatus).

Publication Type:

Journal Article

Source:

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, Volume 23, Issue 4, p.767-79 (2015)

Keywords:

Genomics Core Facility

Abstract:

Centromere sequences exist as gaps in many genome assemblies due to their repetitive nature. Here we take an unbiased approach utilizing centromere protein A (CENP-A) chomatin immunoprecipitation followed by high-throughput sequencing to identify the centromeric repeat sequence in the threespine stickleback fish (Gasterosteus aculeatus). A 186-bp, AT-rich repeat was validated as centromeric using both fluorescence in situ hybridization (FISH) and immunofluorescence combined with FISH (IF-FISH) on interphase nuclei and metaphase spreads. This repeat hybridizes strongly to the centromere on all chromosomes, with the exception of weak hybridization to the Y chromosome. Together, our work provides the first validated sequence information for the threespine stickleback centromere.