Identification of CDK2 substrates in human cell lysates.

Publication Type:

Journal Article

Source:

Genome biology, Volume 9, Issue 10, p.R149 (2008)

Keywords:

2008, Basic Sciences Division, Center-Authored Paper, Clinical Research Division, Cyclin-Dependent Kinase 2, Human Biology Division, Humans, mass spectrometry, Phosphopeptides, PHOSPHORYLATION, Protein Engineering, Ribosomal Proteins, Substrate Specificity

Abstract:

Protein phosphorylation regulates a multitude of biological processes. However, the large number of protein kinases and their substrates generates an enormously complex phosphoproteome. The cyclin-dependent kinases--the CDKs--comprise a class of enzymes that regulate cell cycle progression and play important roles in tumorigenesis. However, despite intense study, only a limited number of mammalian CDK substrates are known. A comprehensive understanding of CDK function requires the identification of their substrate network.