High-throughput sequencing of B- and T-lymphocyte antigen receptors in hematology.

Publication Type:

Journal Article

Authors:

Warren, Edus H; Matsen, Frederick A; Chou, Jeffrey

Source:

Blood, Volume 122, Issue 1, p.19-22 (2013)

Keywords:

2013, Center-Authored Paper, Clinical Research Division, May 2013, Public Health Sciences Division

Abstract:

Application of high-throughput DNA sequencing to the analysis of B- and T-lymphocyte antigen receptors has great potential for improving the monitoring of lymphoid malignancies, assessing immune reconstitution after hematopoietic cell transplantation, and characterizing the composition of lymphocyte repertoires. Current technology can define the number and frequency of IGH, TCRα, TCRβ, or TCRγ chains expressed in a population of lymphocytes; techniques for determining the number of antigen receptor heterodimers, such as TCRαβ pairs, expressed in the population are under development.