Genomic alterations indicate tumor origin and varied metastatic potential of disseminated cells from prostate cancer patients.

Publication Type:

Journal Article

Source:

Cancer research, Volume 68, Issue 14, p.5599-608 (2008)

Keywords:

2008, Cell Adhesion, Cell Line, Tumor, Center-Authored Paper, Chromosome Mapping, DISEASE PROGRESSION, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, GENOME, Genomics Core Facility, Human Biology Division, Humans, In Situ Hybridization, Fluorescence, Male, Models, Biological, Neoplasm Metastasis, Nucleic Acid Hybridization, Prostatic Neoplasms, Public Health Sciences Division, Shared Resources

Abstract:

Disseminated epithelial cells can be isolated from the bone marrow of a far greater fraction of prostate-cancer patients than the fraction of patients who progress to metastatic disease. To provide a better understanding of these cells, we have characterized their genomic alterations. We first present an array comparative genomic hybridization method capable of detecting genomic changes in the small number of disseminated cells (10-20) that can typically be obtained from bone marrow aspirates of prostate-cancer patients. We show multiple regions of copy-number change, including alterations common in prostate cancer, such as 8p loss, 8q gain, and gain encompassing the androgen-receptor gene on Xq, in the disseminated cell pools from 11 metastatic patients. We found fewer and less striking genomic alterations in the 48 pools of disseminated cells from patients with organ-confined disease. However, we identify changes shared by these samples with their corresponding primary tumors and prostate-cancer alterations reported in the literature, evidence that these cells, like those in advanced disease, are disseminated tumor cells (DTC). We also show that DTCs from patients with advanced and localized disease share several abnormalities, including losses containing cell-adhesion genes and alterations reported to associate with progressive disease. These shared alterations might confer the capability to disseminate or establish secondary disease. Overall, the spectrum of genomic deviations is evidence for metastatic capacity in advanced-disease DTCs and for variation in that capacity in DTCs from localized disease. Our analysis lays the foundation for elucidation of the relationship between DTC genomic alterations and progressive prostate cancer.