Genetic analysis of meiotic recombination in Schizosaccharomyces pombe.

Publication Type:

Journal Article

Source:

Methods in molecular biology (Clifton, N.J.), Volume 557, p.65-76 (2009)

Keywords:

2009, Basic Sciences Division, Cell Culture Techniques, Genetic Techniques, MEIOSIS, Models, Biological, Recombination, Genetic, Schizosaccharomyces, Spores, Fungal

Abstract:

The fission yeast Schizosaccharomyces pombe is well-suited for studying meiotic recombination. Methods are described here for culturing S. pombe and for genetic assays ofintragenic recombination (gene conversion), intergenic recombination (crossing-over), and spore viability. Both random spore and tetrad analyses are described.