Gene transfer into humans--immunotherapy of patients with advanced melanoma, using tumor-infiltrating lymphocytes modified by retroviral gene transduction.

Publication Type:

Journal Article

Source:

The New England journal of medicine, Volume 323, Issue 9, p.570-8 (1990)

Keywords:

1990, Adult, Center-Authored Paper, Drug Resistance, Evaluation Studies as Topic, Female, Gene Therapy, Genes, Viral, Genetic Vectors, Humans, IMMUNOTHERAPY, Interleukin-2, LYMPHOCYTES, Male, MELANOMA, Middle Aged, Neomycin, Polymerase Chain Reaction, Retroviridae, Safety, Transfection

Abstract:

Treatment with tumor-infiltrating lymphocytes (TIL) plus interleukin-2 can mediate the regression of metastatic melanoma in approximately half of patients. To optimize this treatment approach and define the in vivo distribution and survival of TIL, we used retroviral-mediated gene transduction to introduce the gene coding for resistance to neomycin into human TIL before their infusion into patients--thus using the new gene as a marker for the infused cells.