Gamma delta+ T cells involvement in viral immune control of chronic human herpesvirus 8 infection.

Publication Type:

Journal Article

Source:

Journal of immunology (Baltimore, Md. : 1950), Volume 180, Issue 5, p.3417-25 (2008)

Keywords:

2008, Adult, Animals, Antibody Development Core Facility, Antiviral Agents, Base Sequence, Cell Differentiation, Cell Line, Transformed, Cell Line, Tumor, Center-Authored Paper, Cercopithecus aethiops, Chronic Disease, Clinical Research Division, Clone Cells, Cytokine Analysis Core Facility, Flow Cytometry Core Facility, Herpesviridae Infections, Herpesvirus 8, Human, Humans, Immunophenotyping, interferon-gamma, Male, Middle Aged, Molecular Sequence Data, Receptors, Antigen, T-Cell, alpha-beta, Receptors, Antigen, T-Cell, gamma-delta, Shared Resources, T-Lymphocyte Subsets, Vero Cells

Abstract:

Little is known about what effector populations are associated with the control of human herpesvirus 8 (HHV-8) infection in vivo. We compared T lymphocyte subsets among HIV-HHV-8+ and HIV-HHV-8- infected human individuals. alphabeta+ T cells from HHV-8-infected individuals displayed a significantly higher percentage of differentiated effector cells among both CD4+ and CD8+ T cell subsets. HHV-8 infection was associated with significant expansion of gammadelta+ Vdelta1 T cells expressing a differentiated effector cell phenotype in peripheral blood. In vitro stimulation of PBMC from HHV-8-infected individuals with either infectious viral particles or different HHV-8 viral proteins resulted in gammadelta Vdelta1 T cell activation. In addition, gammadelta Vdelta1 T cells displayed a strong reactivity against HHV-8-infected cell lines and prevented the release of infectious viral particles following the induction of lyric replication. These data indicate that gammadelta T cells play a role in both innate and adaptive T cell responses against HHV-8 in immunocompetent individuals.