Foamy virus vectors.

Publication Type:

Journal Article

Source:

Journal of virology, Volume 70, Issue 1, p.217-22 (1996)

Keywords:

3T3 Cells, Animals, BLOOD, Cell Division, Cell Line, Cell Line, Transformed, Cercopithecus aethiops, CHO Cells, Cricetinae, Genetic Vectors, Genome, Viral, Humans, Leukemia Virus, Murine, MICE, Rats, Spumavirus, Transduction, Genetic, Vero Cells, Virus Integration

Abstract:

Human foamy virus (HFV) is a retrovirus of the spumavirus family. We have constructed vectors based on HFV that encode neomycin phosphotransferase and alkaline phosphatase. These vectors are able to transduce a wide variety of vertebrate cells by integration of the vector genome. Unlike vectors based on murine leukemia virus, HFV vectors are not inactivated by human serum, and they transduce stationary-phase cultures more efficiently than murine leukemia virus vectors. These properties, as well as their large packaging capacity, make HFV vectors promising gene transfer vehicles.