Expression of a retrovirus encoding human HPRT in mice.

Publication Type:

Journal Article

Source:

Science (New York, N.Y.), Volume 225, Issue 4662, p.630-2 (1984)

Keywords:

362, Animals, Bone Marrow, Bone Marrow Transplantation, DNA, Recombinant, Hematopoietic Stem Cells, Humans, Hypoxanthine Phosphoribosyltransferase, Isoenzymes, Lesch-Nyhan Syndrome, MICE, Nucleic Acid Hybridization, Rats, Retroviridae, Spleen

Abstract:

Transmissible retroviruses encoding human hypoxanthine phosphoribosyltransferase (HPRT) were used to infect mouse bone marrow cells in vitro, and the infected cells were transplanted into mice. Both active human HPRT-protein and chronic HPRT-virus production were detected in hematopoietic tissue of the mice, showing transfer of the gene. These results indicate the possible use of retroviruses for somatic cell therapy.