Expression of a retrovirus encoding human HPRT in mice.

Publication Type:

Journal Article


Science (New York, N.Y.), Volume 225, Issue 4662, p.630-2 (1984)


362, Animals, Bone Marrow, Bone Marrow Transplantation, DNA, Recombinant, Hematopoietic Stem Cells, Humans, Hypoxanthine Phosphoribosyltransferase, Isoenzymes, Lesch-Nyhan Syndrome, MICE, Nucleic Acid Hybridization, Rats, Retroviridae, Spleen


Transmissible retroviruses encoding human hypoxanthine phosphoribosyltransferase (HPRT) were used to infect mouse bone marrow cells in vitro, and the infected cells were transplanted into mice. Both active human HPRT-protein and chronic HPRT-virus production were detected in hematopoietic tissue of the mice, showing transfer of the gene. These results indicate the possible use of retroviruses for somatic cell therapy.