Expression of Prototype Foamy Virus Pol as a Gag-Pol Fusion Protein Does Not Change the Timing of Reverse Transcription.

Publication Type:

Journal Article


Journal of virology (2012)


2012, Basic Sciences Division, Center-Authored Paper, November 2012


Foamy viruses are retroviruses whose Pol protein is synthesized without Gag from a spliced mRNA. Unlike orthoretroviruses, reverse transcription occurs during viral assembly leading to DNA containing virions. When prototype foamy virus Pol is expressed as an orthoretroviral-like Gag-Pol fusion protein, reverse transcription also occurs late in viral replication as measured by the timing of reverse transcriptase sensitivity to the inhibitor AZT. Thus, timing of reverse transcription is intrinsic to Pol itself.