Evaluation of a single round polymerase chain reaction assay using dried blood spots for diagnosis of HIV-1 infection in infants in an African setting.

Publication Type:

Journal Article

Source:

BMC pediatrics, Volume 11, p.18 (2011)

Keywords:

2011, Africa, Center-Authored Paper, DNA, Viral, GENOMICS, HIV Infections, HIV-1, Human Biology Division, Humans, Infant, Polymerase Chain Reaction, Sensitivity and Specificity

Abstract:

The aim of this study was to develop an economical 'in-house' single round polymerase chain reaction (PCR) assay using filter paper-dried blood spots (FP-DBS) for early infant HIV-1 diagnosis and to evaluate its performance in an African setting.