Establishment of long-term tolerance to SRBC in dogs by recombinant canine CTLA4-Ig.

Publication Type:

Journal Article

Source:

Transplantation, Volume 88, Issue 3, p.317-22 (2009)

Keywords:

2009, Animals, Antibodies, Monoclonal, Antibody Development Core Facility, Antibody-Dependent Cell Cytotoxicity, Antigens, CD, Antigens, CD28, Biologics Production Core Facility, Center-Authored Paper, CHO Cells, Clinical Research Division, Comparative Medicine Core Facility, Cricetinae, Cricetulus, Dogs, Erythrocytes, Experimental Histopathology Core Facility, Flow Cytometry Core Facility, hematopoietic stem cell transplantation, Humans, Immune Tolerance, Immunoconjugates, Immunosuppressive Agents, Lymphocyte Culture Test, Mixed, Proteomics Core Facility, Recombinant Fusion Proteins, Shared Resources, Sheep, Time Factors, Transfection

Abstract:

Blockade of the CD28 costimulatory molecule by recombinant human cytotoxic T lymphocyte (CTL)-associated antigen (CTLA4)-Ig or CD40-CD154 interaction with the monoclonal antibody 5C8 together with donor-specific transfusion led to enhanced engraftment in the canine model of dog leukocyte antigen (DLA)-identical marrow transplantation after 1 Gy total body irradiation. To reduce or eliminate total body irradiation conditioning regimens, we have sought to develop canine specific reagents.