Electrospray mass spectrometry for quantitative plasma proteome analysis.

Publication Type:

Journal Article

Source:

Methods in molecular biology (Clifton, N.J.), Volume 564, p.227-42 (2009)

Keywords:

2009, Blood Proteins, Center-Authored Paper, Chromatography, Affinity, Chromatography, Liquid, Electrophoresis, Gel, Two-Dimensional, Humans, Peptide Fragments, Peptide Mapping, Proteome, Public Health Sciences Division, Spectrometry, Mass, Electrospray Ionization

Abstract:

Electrospray ionization mass spectrometry (ESI-MS) is an efficient soft ionization procedure for macro biomolecules. However, it is a rather delicate process to produce charged molecules for mass-to-charge ratio (m/z) based measurement. In this chapter, the mechanism of ESI is briefly presented, and the experimental pipeline for quantitative profiling of plasma proteins (prefractionation immunodepletion, protein isotope tagging, 2D-HPLC separation of intact proteins, and LC-MS) is presented as applied by our group in studies of cancer biomarker discovery.