DNA-damaging agents greatly increase the transduction of nondividing cells by adeno-associated virus vectors.

Publication Type:

Journal Article


Journal of virology, Volume 68, Issue 12, p.8282-7 (1994)


1994, Alkaline Phosphatase, beta-Galactosidase, Cell Line, Cells, Cultured, Cesium, Cisplatin, Dependovirus, DNA Damage, Gamma Rays, gene expression, Genetic Vectors, Hela Cells, Humans, Infant, Newborn, Kinetics, Male, methotrexate, Nocodazole, Skin, Transduction, Genetic


None of the vector systems currently available for gene therapy applications have been shown to be capable of both efficient gene transfer into nondividing cells and long-term expression through stable integration into host cell DNA. While integrating vectors based on adeno-associated virus are capable of mediating gene transfer into nondividing cells, this process is 200-fold less efficient than transduction of dividing cells. We demonstrate that the transduction efficiency of adeno-associated virus vectors can be increased by treatment with DNA-damaging agents. Nondividing cells are especially responsive, with increases in transduction efficiency of up to 750-fold. This finding has the potential to facilitate gene therapy applications requiring gene transfer to nondividing cells.