Differentiation of erythroleukemia cells in vitro: properties of chemical inducers.

Publication Type:

Journal Article


Cell differentiation, Volume 4, Issue 3, p.179-85 (1975)


Acetamides, Acetone, Animals, BLOOD, Cell Differentiation, Cell Line, Cryoprotective Agents, Culture Media, Dialysis, Dimethyl Sulfoxide, Drug Interactions, Drug Synergism, Ethylene Glycols, Formamides, Heme, Leukemia, Erythroblastic, Acute, MICE, Molecular Weight, Pharmaceutical Preparations, Pyridines, Urea


Murine erythroleukemia cells growing in vitro can be induced to differentiate. The physical properties of chemical inducers has been defined and has permitted the recognition of a variety of potent inducers. The compounds can be linear, branched, or aromatic molecules. Some are methylated. All are low molecular weight basic compounds which are hydrophylic and are potent cryoprotective agents. The inducing effects of combinations of some agents are synergistic while others are additive. These observations will permit a rational search for inducing agents with increased potency and decreased toxicity.