Development of a reliable low-cost controlled cooling rate instrument for the cryopreservation of hematopoietic stem cells.

Publication Type:

Journal Article


Cytotherapy, Volume 12, Issue 2, p.161-9 (2010)


2010, Antigens, CD34, Cell Processing Core Facility, Cell Survival, Center-Authored Paper, Clinical Research Division, Cold Temperature, Colony-Forming Units Assay, Cryopreservation, Erythroid Precursor Cells, Flow Cytometry Core Facility, Granulocyte-Macrophage Progenitor Cells, Hematopoietic Stem Cells, Humans, Models, Biological, Shared Resources


An optimal cooling rate is one of the critical factors influencing the survival of cells during cryopreservation. We describe a novel device, called the box-in-box, that has been developed for optimal cryopreservation of human hematopoietic stem cells (HSC).