Development and optimization of quantitative PCR for the diagnosis of invasive aspergillosis with bronchoalveolar lavage fluid.

Publication Type:

Journal Article


BMC infectious diseases, Volume 8, p.73 (2008)


2008, Adolescent, Adult, Aged, Aspergillosis, Aspergillus, Bronchoalveolar Lavage Fluid, Center-Authored Paper, Clinical Research Division, DNA, Fungal, Female, Genomics Core Facility, Humans, Lung Diseases, Fungal, Male, Middle Aged, Polymerase Chain Reaction, Reproducibility of Results, Retrospective Studies, Sensitivity and Specificity, Shared Resources, Vaccine and Infectious Disease Institute


The diagnosis of invasive pulmonary aspergillosis (IPA) remains challenging. Culture and histopathological examination of bronchoalveolar lavage (BAL) fluid are useful but have suboptimal sensitivity and in the case of culture may require several days for fungal growth to be evident. Detection of Aspergillus DNA in BAL fluid by quantitative PCR (qPCR) offers the potential for earlier diagnosis and higher sensitivity. It is important to adopt quality control measures in PCR assays to address false positives and negatives which can hinder accurate evaluation of diagnostic performance.