Coupling endonucleases with DNA end-processing enzymes to drive gene disruption.

Publication Type:

Journal Article


Nature methods, Volume 9, Issue 10, p.973-5 (2012)


2012, Clinical Research Division, September 2012


Targeted DNA double-strand breaks introduced by rare-cleaving designer endonucleases can be harnessed for gene disruption applications by engaging mutagenic nonhomologous end-joining DNA repair pathways. However, endonuclease-mediated DNA breaks are often subject to precise repair, which limits the efficiency of targeted genome editing. To address this issue, we coupled designer endonucleases to DNA end-processing enzymes to drive mutagenic break resolution, achieving up to 25-fold enhancements in gene disruption rates.