Correlation of mRNA and protein levels: cell type-specific gene expression of cluster designation antigens in the prostate.

Publication Type:

Journal Article

Authors:

Pascal, Laura E; True, Lawrence D; Campbell, David S; Deutsch, Eric W; Risk, Michael; Coleman, Ilsa M; Eichner, Lillian J; Nelson, Peter S; Liu, Alvin Y

Source:

BMC genomics, Volume 9, p.246 (2008)

Keywords:

2008, Adult Stem Cells, Antigens, CD, Cell Separation, Center-Authored Paper, Endothelial Cells, Epithelial Cells, gene expression, Gene Expression Profiling, Genomics Core Facility, Human Biology Division, Humans, Immunohistochemistry, Male, Oligonucleotide Array Sequence Analysis, prostate, Prostatic Neoplasms, RNA, Messenger, RNA, Neoplasm, Shared Resources, Stromal Cells

Abstract:

: Expression levels of mRNA and protein by cell types exhibit a range of correlations for different genes. In this study, we compared levels of mRNA abundance for several cluster designation (CD) genes determined by gene arrays using magnetic sorted and laser-capture microdissected human prostate cells with levels of expression of the respective CD proteins determined by immunohistochemical staining in the major cell types of the prostate - basal epithelial, luminal epithelial, stromal fibromuscular, and endothelial - and for prostate precursor/stem cells and prostate carcinoma cells. Immunohistochemical stains of prostate tissues from more than 50 patients were scored for informative CD antigen expression and compared with cell-type specific transcriptomes.