Cop9 signalosome subunit 8 (CSN8) is essential for Drosophila development.

Publication Type:

Journal Article

Source:

Genes to cells : devoted to molecular & cellular mechanisms, Volume 13, Issue 3, p.221-31 (2008)

Keywords:

2008, Animals, Animals, Genetically Modified, Basic Sciences Division, Center-Authored Paper, DROSOPHILA, Drosophila Proteins, Flow Cytometry Core Facility, Gene Targeting, Genes, Lethal, Genomics Core Facility, Intracellular Signaling Peptides and Proteins, Larva, Life Cycle Stages, MEIOSIS, MELANOMA, Multiprotein Complexes, Mutation, Nuclear Proteins, Oogenesis, Peptide Hydrolases, PHENOTYPE, Research Trials Office Core Facility - Biostatistics Service, Reverse Transcriptase Polymerase Chain Reaction, RNA, Messenger, Scientific Imaging Core Facility, Shared Resources

Abstract:

The COP9 signalosome (CSN) is a multisubunit regulator highly conserved in evolution. We show here that CSN subunit 8 (CSN8) is essential for Drosophila development. CSN8 is maternally contributed and present throughout development. Null mutants generated in this study are larval lethal, showing phenotypes associated with mutations in either CSN4 (molting defects) or CSN5 (melanotic tumors). Analysis of mitotic and germ-line csn8(null) clones revealed the requirement of CSN8 for multiple developmental processes. The germ-line clones arrested at mid-oogenesis, while the mitotic clones led to deformed adult eyes or wings. CSN8 is present exclusively as part of the CSN holo-complex, and lack of CSN8 in the mutants leads to CSN instability. Consistent with this, Cullin deneddylation is impaired in the csn8(null) mutants.