Control of G2 delay by the rad9 gene of Saccharomyces cerevisiae.

Publication Type:

Journal Article

Source:

Journal of cell science. Supplement, Volume 12, p.145-8 (1989)

Keywords:

DNA Damage, DNA Replication, DNA, Fungal, Genes, Fungal, Interphase, Mitosis, Mutation, Saccharomyces cerevisiae

Abstract:

In response to DNA damage, Saccharomyces cerevisiae cells arrest the cell cycle in the G2 phase. Arrest is defective in rad9 mutants; rad9 cells divide and die without repairing the damage. Several cell cycle mutants that are defective in DNA replication arrest in G2 at the restrictive temperature; this arrest is due to the RAD9 control function. Thus RAD9 is responsible for the fact that mitosis is normally dependent upon DNA replication, a function we term a 'checkpoint'. Four additional genes have been identified that are also components of the RAD9 checkpoint.