The conserved HDAC Rpd3 drives transcriptional quiescence in S. cerevisiae.

Publication Type:

Journal Article

Source:

Genomics data, Volume 6, p.245-8 (2015)

Keywords:

Flow Cytometry Core Facility, Genomics Core Facility

Abstract:

Quiescence is a ubiquitous cell cycle stage conserved from microbes through humans and is essential to normal cellular function and response to changing environmental conditions. We recently reported a massive repressive event associated with quiescence in Saccharomyces cerevisiae, where Rpd3 establishes repressive chromatin structure that drives transcriptional shutoff [6]. Here, we describe in detail the experimental procedures, data collection, and data analysis related to our characterization of transcriptional quiescence in budding yeast (GEO: GSE67151). Our results provide a bona fide molecular event driven by widespread changes in chromatin structure through action of Rpd3 that distinguishes quiescence as a unique cell cycle stage in S. cerevisiae.