Assessment of mitochondrial toxicity by analysis of mitochondrial protein expression in mononuclear cells.

Publication Type:

Journal Article

Source:

Cytometry. Part B, Clinical cytometry, Volume 76, Issue 3, p.181-90 (2009)

Keywords:

2009, Adenosine Triphosphatases, Adult, Child, Child, Preschool, Cyclooxygenase 1, DNA, Mitochondrial, Dose-Response Relationship, Drug, Ethidium, Flow Cytometry Core Facility, Humans, Infant, Leukocytes, Mononuclear, Middle Aged, mitochondria, Mitochondrial Proteins, Reproducibility of Results, Sensitivity and Specificity, Vaccine and Infectious Disease Institute, Young Adult, Zalcitabine

Abstract:

Real-time PCR has quantified decreased mitochondrial DNA levels in association with nucleoside reverse transcriptase inhibitor (NRTI) therapy of HIV-infected populations. However, real-time PCR is best suited to distinguish log differences in an analyte. In an effort to monitor individuals in more detail, we developed a flow cytometric assay to gauge mitochondrial function.